1321N1 (+Gα16) AequoScreen® Parental cell line

Manufacturer PerkinElmer, Inc.

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AequoScreen® Parental cells stably expressing the mitochondrially targeted apoaequorin and the G-protein Gα16 for flash-luminescence assay.
Recombinant, in 1321N1 host cell. GPCRs of your choice can be transiently / stably transfected into these cell lines. Following GPCR stimulation, increases in intracellular calcium enable measurement of the resulting flash luminescence signal. Two vials of cryopreserved cells are shipped per order.


  • Functional, homogenous and generic assay platform for Gs and Gi coupled GPCRs
  • Fully automatable and miniaturizable to 1536
  • Enables reduction in reagents and consumable costs
  • High signal to background ratio enabling detection of agonists, antagonists and allosteric modulators
  • Robust assay (Z, CV values, solvent resistance)
  • Reduces the number of false positives from autofluorescent compounds in screening libraries
  • Option to work with cells in suspension to increase throughput

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1321N1 (+Gα16) AequoScreen® Parental cell line

Manufacturer PerkinElmer, Inc.

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