1260 Infinity Nanoflow LC by Agilent Technologies
Manufacturer Agilent Technologies
Unmatched low-flow LC performance and stability
1260 Infinity Nanoflow LC
Average Rating: 2.0
1 Scientist has reviewed this product
Status:
Reviewer
Member since: 2013
Organization: Department of Analytical Chemistry, University of Valencia
-
- Ease of use
- After sales service
- Value for money
Rating: 2.0
Application Area: I'm using this product for separation of small molecules
"The Agilent nanoLC is a binary pump with four channels that has a split. This split is a headache, because you need to take into account the flow of the primary part to avoid errors in your chromatograms. In addition, the autosampler with 8 microliters obligate you to do a complicated program of injection that includes several changes of the valve to eliminate the big internal volume when you are going to inject about 20 nL. The best part probably, is the detector; however, you need to change the included cell (with a cell volume too big) for a cell of 5 nL from Sunchrom. This cell from Sunchrom is really good until you have problems with the capillaries. The capillaries are soft and easy to break, no changes are possible, and so you need to change the cell, which is really expensive. Ultimately the instrument with more work on it will be a good alternative for nanoLC systems, however nowadays is really complicated to work with it in separation of molecules by direct injection. "
Review date: 25 Feb 2015 | 1260 Infinity Nanoflow LC
Unmatched low-flow LC performance and stability
The 1260 Infinity Nanoflow LC features Electronic Flow Control (EFC) with active feedback and real-time flow adjustment. EFC is a unique Agilent technology and delivers a constant flow to the column that is independent of system backpressure. Precise flow rates support stable ion generation to ensure you obtain high-sensitivity nanoflow LC/MS performance.
Features:
- Unsurpassed low-flow performance and stability - the nanoflow pump features proven Electronic Flow Control (EFC) with active feedback for unprecedented stability at flow rates from 100 nl/min to 1 µl/min.
- Reproducible retention times - EFC with real-time flow control delivers a constant nanoflow that is independent of system back pressure, so retention times are highly reproducible and nanospray operation is extremely stable.
- Flexibility for non-nano applications - in addition to nanoflow operations, the system offers full gradient capability for up to 2.5 ml/min in 'normal mode' adding to the system's versatility.
- Maximum separation efficiency - the switching valves, fittings, connectors and PEEK-coated fused silica tubing have all been carefully selected to minimize dead volumes.
- Automated sample cleanup, enrichment and 2D HPLC with conventional nanocolumns.
- Conversion to 2D HPLC by adding another column (e.g. ion exchange column).
- Easy-to-operate nanospray LC/MS with the innovative and fully integrated HPLC-Chip/MS system.
- Key component of the Agilent Nanospray Protein ID solution and the HPLC-Chip/MS Protein ID solution.
- Fully compatible with all Agilent mass spectrometers and with most third party ESI, nanospray or MALDI MS systems. Versatile software to control several platforms.
Product Overview
