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Member since: 2013
Organization: Department of Analytical Chemistry, University of Valencia
Application Area: I'm using this product for separation of small molecules
"The Agilent nanoLC is a binary pump with four channels that has a split. This split is a headache, because you need to take into account the flow of the primary part to avoid errors in your chromatograms. In addition, the autosampler with 8 microliters obligate you to do a complicated program of injection that includes several changes of the valve to eliminate the big internal volume when you are going to inject about 20 nL. The best part probably, is the detector; however, you need to change the included cell (with a cell volume too big) for a cell of 5 nL from Sunchrom. This cell from Sunchrom is really good until you have problems with the capillaries. The capillaries are soft and easy to break, no changes are possible, and so you need to change the cell, which is really expensive. Ultimately the instrument with more work on it will be a good alternative for nanoLC systems, however nowadays is really complicated to work with it in separation of molecules by direct injection. "