E-Gel® 96 gels are bufferless, pre-cast agarose gels designed for fast, high-throughput DNA electrophoresis. Each gel contains 96 sample lanes and 8 marker lanes that are ideal for screening multiple PCR products, plasmid preparations, and restriction digests. The unique 96-well staggered-well format provides a 1.6-cm run length that resolves between 100 bp and 10 kb. The E-Gel® 96 loading format is compatible with multi-channel pipettors, and the most commonly used 8-, 12-, and 96-pin liquid handling robots (Figure 1). With 12-minute run times, up to 20,000 samples can be resolved in a single day.