Candida albicans IgA ELISA by DRG International Inc.

An enzyme immunoassay for the qualitative and semiquantitative determination of IgA-class antibodies to Candida albicans in serum. The DRG Candida albicans IgA ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) Microtiter wells as a solid phase are coated with Candida albicansantigen. Diluted patient specimens and ready-for-use controls are pipetted into these wells. During incubation Candida albicans-specific antibodies of positive specimens and controls are bound to the immobilized antigens. After a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human IgA antibodies are dispensed into the wells. During a second incubation this anti-IgA conjugate binds specifically to IgA antibodies resulting in the formation of enzyme-linkedimmune complexes. After a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid. The intensity of this color is directlyproportional to the amount of Candida albicans-specific IgA antibody in the patient specimen.  Absorbance at 450 nm is read using an ELISA microtiter plate reader.