Heck: “The impact of the high mass resolving power at very high sensitivity as achievable with this new mass spectrometer is tremendous; it opens up avenues to measure not only protein-protein interactions, but also covalent and non-covalent binding of small molecules to protein assemblies.
Wide-ranging applications may include the direct analysis of drug molecules binding to their targets, and the investigation of post-translational and chemical modifications (e.g. phosphorylation, glycosylation) on intact proteins and protein assemblies. I foresee that this instrument will become instrumental in the development and use of therapeutic antibodies, but also for instance in analyzing how drug molecules such as proteasome inhibitors do interact with their target, the proteasome”.
Makarov: “Presently Orbitrap mass spectrometry is probably the fastest growing mass spectrometric technique. Through this collaboration with Utrecht University we have opened up new avenues for the use of this mass analyzer. I always believed in the versatility of the Orbitrap analyzer, but am still amazed to see that we can now also mass analyze huge protein complexes, even whole viruses, with substantially improved resolving power and mass accuracy and sensitivity down to individual ions.”
This research was made possible by support of the Netherlands Proteomics Centre, and the EU-funded large scale facility PRIME-XS.