Celebrating 10 years of PCR: High-Fidelity PCR Reagents Continue to Propel DNA Research with Unparalleled Accuracy and Speed

14 Aug 2013
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New proprietary tracking dyes within high-fidelity DNA polymerase and qPCR master mixes facilitate easier, more accurate PCR set-up for researchers.

The Thermo Scientific Phusion Green High-Fidelity DNA Polymerase and Luminaris Color qPCR master mixes utilize proprietary tracking dyes to speed up the pipetting process. By providing a visual confirmation, the mixes are designed to vastly reduce the chance of human error, while also decreasing the number of procedural steps required. Most importantly, the dyes have been engineered not to interfere with the reaction or any downstream applications.

These new Phusion and Luminaris products form the latest additions to the renowned Thermo Scientific Phusion High-Fidelity DNA Polymerase family. First launched a decade ago, the Phusion High-Fidelity DNA Polymerase was the first DNA polymerase, developed using fusion protein technology to provide a combination of accuracy and speed previously unattainable using conventional enzymes.

Known for performance with all major PCR applications, the Phusion technology has played a central role in helping researchers accomplish an array of key scientific achievements, including the creation of the first functional synthetic genome.

“Phusion Polymerase technology has dramatically increased the productivity of labs running PCR,” says Margarita Leckiene, Director of Nucleic Acid Detection & Molecular Tools for Thermo Fisher Scientific. “Our philosophy is to simplify customer workflows further and increase the reproducibility of results. We are constantly working to further advance the accuracy and robustness of Phusion polymerase including the most recent innovation of special enzyme formulations that are optimized for NGS applications, offering equal amplification efficiency across entire genomes.”

This technology, coupled with Thermo Scientific PCR instruments and accessories such as the Thermo Scientific Arktik Thermal Cycler and Thermo Scientific Piko Plate Illuminator, provides an efficient integrated solution that delivers:

• DNA amplification with extreme accuracy
• Enhanced visual control
• Significantly shorter protocol times

To recognize the impact of the original Phusion polymerase and a subsequent decade of innovation in this area, Thermo Fisher has launched Phusion Fest, an interactive campaign celebrating the importance of PCR in the laboratory. Phusion Fest encompasses a fun, scientific trivia game, special offers across a variety of molecular biology products and free samples of selected Phusion products.

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