This ELISA kit enables sensitive identification and quantification of APP ΔC31, detecting as little as 0.92 pM of APP ΔC31 in human cell lysate and cerebral spinal fluid samples. The assay provides quantitative results with excellent reproducibility between experiments, offers greater efficiency over Western blotting by requiring less sample input, and provides analysis of up to 38 duplicate samples in just 2 hours. The ELISA displays high specificity to human APP ΔC31 with low cross reactivity to other APP isoforms. The assay was developed in collaboration with the Buck Institute for Research on Aging and with the support of a grant from the NIA (grant # AG041456). Researchers at the Buck Institute have validated use of the assay in drug screening applications for caspase inducers and caspase inhibitors.
Flexible design
Like all Enzo Life Sciences ELISA kits, the APP ΔC31 ELISA kit is complete, flexible, and put through rigorous fit-for-purpose validation and stability testing to ensure high precision, accuracy, sensitivity, and specificity. It is supplied with an easy-to-follow protocol, pre-coated microtiter plate, and color-coded liquid reagents to save time and reduce errors. The 96-well break-apart strip plate design provides throughput flexibility to minimize waste.
The APP ΔC31 ELISA kit is the most recent addition to Enzo’s industry-leading portfolio of products for neurodegenerative disease, including products for detection of Tau protein, &beta:-amyloid and 24(S) Hydroxycholesterol.