Agilent Technologies Inc. introduced a new High Sensitivity DNA Kit for quality control, sizing and quantification of fragmented DNA and DNA libraries in the Next Generation Sequencing (NGS) workflow. The kit is used with the Agilent 2100 Bioanalyzer, the most successful microfluidics-based platform available commercially, offering solutions for the analysis of DNA, RNA, proteins and cells.
Agilent Technologies Introduces High Sensitivity DNA Kit Targeting DNA, DNA Libraries in Next Generation Sequencing Workflow
With its high sensitivity the kit will help NGS users working with double-stranded DNA to increase the fidelity of their sequencing data. It enables users to quantify DNA fragments in the lower single-digit pg/µl concentration range. Many NGS sample-preparation protocols require DNA library amplification by PCR, which can often introduce amplification bias. The high sensitivity significantly lowers the number of required PCR cycles, reducing amplification artefacts and improving the quality of sequencing data.
“This is the most sensitive DNA detection method of all gel- and microfluidics-based systems currently on the market,” said Knut Wintergerst, electrophoresis marketing and support manager at Agilent. “The advent of NGS has dramatically changed the genetic landscape by significantly decreasing costs of a whole genome sequencing project while increasing experimental speed. Researchers need high quality DNA data quickly and Agilent has created a kit that provides just that.”
As the popularity of NGS platforms continues to rise and the premise of Pharmacogenomics continues to evolve, increasing the fidelity of NGS data is a crucial aspect of the workflow for researchers. With its ease of use, reliability, versatility – single stranded DNA QC and RNA QC in NGS workflows – and throughput of 12 samples in 30 minutes, the Agilent 2100 bioanalyzer is an ideal QC tool for NGS platforms.
Launched in 1999, the Agilent 2100 Bioanalyzer was the first commercially available instrument to use microfluidics technology for the analysis of biological samples. The 2100 Bioanalyzer can be used for electrophoresis and flow cytometry applications.
Company websiteAgilent Technologies