In order to accommodate the narrow peaks produced by UHPLC separation, Wyatt Technology’s µDAWN engineers reduced the conventional light scattering flow cell volume from 63 µL to fewer than 10µL. More importantly, a remarkable improvement was achieved in minimizing interdetector mixing; the band broadening between the µDAWN MALS and Optilab UT-rEX detectors was brought to under 7 µL, while the band broadening between the UHPLC’s UV detector and the µDAWN detector is a mere 2 µL! As a consequence of the reduced cell volume and interdetector band broadening, a µDAWN/UT-rEX system can accurately analyze the molar mass and size of UHPLC peaks without loss of resolution.
Reduction in the cell volume of a MALS system can create significant technical difficulties due to the increased proximity of the laser beam to cell/sample interfaces. This closeness leads to higher levels of stray light which can compromise instrument sensitivity. Innovations in the µDAWN’s detection optics and mechano-optical train not only maintain the sensitivity level but actually exceed that of Wyatt’s popular miniDAWN TREOS MALS detector.
Further expanding the versatility of the µDAWN is the WyattQELS™ Dynamic Light Scattering (DLS) module. This embedded accessory measures DLS in the µDAWN flow cell simultaneously with MALS acquisition, computing hydrodynamic radii “on-the-fly”. And finally, the µDAWN user experience is augmented by its color touchscreen front panel display which shows the chromatographic conditions, instrument diagnostics, and much more.
To learn more about Wyatt Technology, please visit booth #3554 at Pittcon 2014. Alternatively please click on the company website or more info link below.