Valuable research in the field of neuroscience
"The value of this work to the neuroscience community is evident in the attention it attracted online, ranking second overall for Nature Methods," says Orla Hanrahan of Andor. "Because their imaging methodology can also be applied straightforwardly to other organisms, or more general imaging tasks, and since their novel calcium reporter is a very powerful tool that can be used by all neuroscientists working on C. elegans, their technique has already had a large impact. If a lab-on-a-chip device is deployed for stimulus delivery in the future, this method provides an enabling platform for establishing functional maps of neuronal networks."
Unlike previous CMOS or CCD technologies, the Andor Neo 5.5 sets radical new benchmarks in its unique ability to simultaneously deliver highest specifications in sensitivity, resolution, speed, dynamic range and field-of-view. The camera is based around a large 5.5 megapixel sensor with 6.5 µm pixels and a 22 mm diameter and is capable of 30 fps sustained or up to 100 fps burst to the 4 GB internal memory, making it ideal for applications in cell microscopy, astronomy, digital pathology, and high content screening. Built on a -40°C vacuum-cooled platform, the extremely low darkcurrent and 1 e- read noise means Neo 5.5 is suited to a range of exposure conditions. The Rolling and Global shutter flexibility further enhances application flexibility, Global shutter in particular offering an ideal means to simply and efficiently synchronize the Neo with other 'moving' devices, such as stages or light switching sources, and eliminating the possibility of spatial distortion when imaging fast moving objects.
About the Vaziri and Zimmer Groups
The Vaziri group's research focuses on design and exploration of novel microscopy methods with a particular interest in neuro-scientific applications, such as fast volumetric imaging of neuronal activity. The Zimmer group focuses on neural circuits involved in chemo-sensing in C. elegans. In their recent Nature Methods paper, these two research directions were joined to deliver an exciting platform for demonstrating the capabilities and power of modern microscopy techniques.
• Tina Schrödel, Robert Prevedel, Karin Aumayr, Manuel Zimmer and Alipasha Vaziri "Brain-wide 3D imaging of neuronal activity in Caenorhabditis elegans with sculpted light" Nature Methods Vol. 10, 1013-1020 (2013)