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The assay involves two monoclonal antibodies, one labeled with Cryptate and the other with XL665, that recognize distinct determinants. The assay is performed in a single step and requires only a short incubation period. Detection limit ... Read more...
Universal Assay for Serine/Threonine Kinases Cisbio's line of HTRF ® KinEASE™ STK kits are based on our patented HTRF ® technology and can be used as a universal tool for assessing Serine/Threonine kinase activity. These assays use a ... Read more...
This HTRF kit detects changes of cAMP accumulation in response to Gs coupled GPCR activation or inhibition. Read more...
Cisbio offers a line of three ready-to-use assays for measuring cAMP using HTRF® technology. Each kit is optimized for detecting a specific range of cAMP concentrations to allow fine tuning of assay sensitivity. These kits enable high qual... Read more...
The Human IgG kit is designed for the fast quantification of human IgG. Read more...
Three kits to cover a wide range of cAMP concentrations. After the introduction of IP-One, the first HTS immunoassay that can easily detect inositol(1)phosphate (IP1), a stable second messenger which tightly correlates with Gq-coupled r... Read more...
An easy way to assess tag accessibility The production of fusion proteins often raises the issues not only of their structural integrity after isolation, but also of the accessibility of the associated tag. In the case of GST or 6HIS tag... Read more...
A rapid and sensitive homogeneous assay for cortisol assessment The HTRF® cortisol assay can be used for the quantification of cortisol in rodent serum, or directly on cells. This assay has also been validated for 11-beta HSD1 screening... Read more...
A straightforward tool for biomolecular labeling with Europium cryptate The Cryptate labeling kit includes all the necessary materials to allow rapid Cryptate labeling of various biomolecules and purification. The labeling reagent Eu3+-... Read more...
A range of HTRF® conjugates for assay development HTRF® toolbox assembles a panel of detecting reagents such as Streptavidin-XLlent!, anti-tag antibodies like Eu-Cryptate anti-GST, 6HIS, DNP and anti species conjugated antibodies offer... Read more...
A simple and straightforward procedure for measuring functional kinase activity. The Eu(K) labeled antibody and XL665 conjugate are added simultaneously after the enzymatic step. Results are available after a 2-hour incubation at room... Read more...
An homogeneous fluorescence assay for PGE2 Prostaglandin E2 (PGE2) is a primary product of arachidonic metabolism in many cells. Under inflammatory stimuli, PGE2 is de novo synthesized by three enzymatic steps via the cyclooxygenase (CO... Read more...
Cisbio's HTRF ® KinEASE™ platform has expanded to include a universal assay for tyrosine kinases. The HTRF KinEASE TK assay is based on our patented HTRF technology and uses a unique tyrosine peptide substrate in combination with a Europi... Read more...
IP-One ELISA assay kit from Cisbio Bioassays - Simple and highly accurate measurement of IP1 IP1, a downstream metabolite of IP3, accumulates in cells following activation of Phospholipase C (PLC) coupled receptors. Among them, t... Read more...
Do you - have trouble finding an assay that fits your needs perfectly or do you want to convert your ELISA into an homogeneous method? Through Assays on demand, Cisbio gives you a list of its latest developments for the Drug Discovery ... Read more...
ADP detection with HTRF® quality. The HTRF® Transcreener™ ADP assay is a homogeneous non-radioactive assay which measures the generation of ADP. This universal solution can be used to assess kinase and ATPase activity for High Through... Read more...
Streamlined single plate protocols including all stimulation and cytokine quantification. The HTRFâ cytokine kit line includes TNFa, IFNg, IL1b, IL6 and IL8 assays. These assays feature no separation steps, a high sensitivity and dynami... Read more...
A homogeneous single plate assay for the sensitive quantification of histamine. Histamine is a small biogenic amine that plays an important role in several physiological and pathophysiological processes. The histamine HTRF® assay is bas... Read more...
A streamlined assay for human monoclonal antibody screening. The human Fc check kit is a competitive immunoassay involving a Cryptate conjugated polyclonal antibody specific to human Fc IgGs and XL665 coupled human IgGs. This homogeneou... Read more...
A new secondary messenger for Gq coupled receptor investigation. Cisbio’s IP-One assay, based on its proprietary HTRF® technology, is the first HTS immunoassay that can easily detect inositol(1)phosphate (IP1), a stable second messenger... Read more...
A unique set of reagents for the investigation of ubiquitin. The HTRF® Ubiquitin toolbox assembles all the reagents for flexible assay development and HTS of each step of the ubiquitin system. Ubiquitin is directly labeled with the Eu3+... Read more...
A team of experts for outsourcing your assay developments Our Service Team at Cisbio international is highly trained in protein chemistry and fluorescent technology, and offers you its expertise in assay development to build HTRF® assay... Read more...
A fast and reliable process for biomolecule labeling In many circumstances, assay development and HTS involve specific labeling of the various assay components. Cisbio international’s labeling service is an excellent alternative for the... Read more...
Cisbio's cAMP HiRange kit is designed to address either the use of cell-based and cellular membranes-assays in GPCR activation and Adenylate Cyclase activity screening. The cAMP HiRange assay is highly robust and produces quality results w... Read more...
IP1, a downstream metabolite of IP3, accumulates in cells following Gq recepetor activation and is stable in the presence of LiCl making it an ideal GPCR (Gq) functional assay. Cisbio developed and patented a highly accurate HTRF assay for... Read more...