Merck Products

Sigma-Aldrich ® one-part sgRNA and two-part crRNA:tracrRNA systems accelerate genome editing with Cas9 protein, mRNA, or established Cas9 expressing cell lines. Our guides are compatible with a variety of delivery methods including m... Read more...

With the largest portfolio of Cas9 proteins available, Merck make it easy to find the perfect nuclease for your gene editing experiment. Read more...

Nuclease-independent applications of CRISPR provide equal targeting specificity but instead of cutting, CRISPRi allows for targeted interference of gene function by delivering transcriptional repressor domains to a specific target sequenc... Read more...

The next generation of RNP reagents Read more...

The T7 Endonuclease Detection Assay is a well-known method for detecting genome editing events from CRISPR, Zinc-finger nuclease, and TALEN gene targeting. Originally identified from Escherichia coli bacteriophage, the T7 endonuclease can... Read more...

All-in-one, ready-to-use Cas9 and guide RNA (gRNA) expression plasmids for use with monocots and dicots. Read more...

CRISPR/Cas9-mediated recombineering is the most powerful bacterial genome engineering method to date. In addition, Cas9-mediated recombineering overcomes the dependence on a second recombination step, avoids the creation of destabilizing ... Read more...

This Lentiviral Packaging Mix is an optimized formulation of two plasmids expressing the key HIV packaging genes and a heterologous viral envelope gene. Read more...

Universal negative control CRISPRs have been designed not to recognize any sequence in the human, mouse or rat genome.  Read more...

Compare and contrast the features of a wide variety of guide RNA (gRNA) and Cas9 products for in vitro and in vivo CRISPR experiments. Select the best formats for your mammalian or plant applications: SygRNA ® synthetic gRNA, plasmid ... Read more...

Exclusive human or mouse whole genome arrayed libraries developed with the Wellcome Sanger institute Read more...

Pooled CRISPR screening with 10x Genomics compatibility Read more...

Recombinant PURedit Cas9 protein from Streptococcus pyogenes is a ready-to-use reagent for genome engineering experiments Read more...

Recombinant Cas9 Plus protein from Streptococcus pyogenes is a ready-to-use reagent for genome engineering experiments. Read more...

Recombinant Cas9-GFP protein from Streptococcus pyogenes is a ready-to-use reagent for genome engineering experiments. Read more...

The human CRISPR ′Brunello′ pooled library is designed using optimized metrics, as published by, Doench et al. Nat Biotechnol. (2016) and described further in Sanson, K.R., et al. Nat Commun (2018) Read more...

The Human Whole Genome Toronto KnockOut Library v3 (TKOv3) is optimized for editing efficiency using empirical data described in the findings by the Moffat lab. Read more...

The human, paired guide Poison (pgPoison) library targets 3` splice sites with paired guide RNAs for alternative exon removal (pgRNAs), induce skipping of "poison" cassette exons and corresponding upstream constitutive exons in ... Read more...

The mouse CRISPR "Brie" lentiviral pooled libraries are designed using optimized metrics, as published by Sanson, K.R., et al. Nat Commun 9, 5416 (2018), which combine improved on-target activity predictions (Rule Set 2) with an... Read more...

The Human CRISPR inhibition (CRISPRi) library (Dolcetto) is a single compact library that inhibits over 18,000 human genes and is used for genome-wide inhibition screening using a KRAB-dCas9 effector. Read more...

The 10x Compatible Human CRISPRi Kinase Phosphatase Drug Targets Kit (Druggable Genome) contains one sub-pool of the top two ranked sgRNAs per gene for increased sensitivity and includes non-targeting controls built-in. Read more...

The Human CRISPR activation library (Calabrese) activates over 18,000 human genes and is used for genome-wide activation screening. The library is designed to be compact and efficient to maximize screening efficiency and performance, as p... Read more...

The mouse CRISPR activation library (Caprano) activates over 22,000 mouse genes and is used for genome-wide activation screening. Read more...

Design gRNA or search pre-designed guides Read more...

The control kit includes validated positive control targeting RAB1A and non-targeting control for assay set-up and a UCOE KRAB-dCas9 for consistent effector expression across a wide variety of cell lines. The kit is ideal for setting up t... Read more...