Vector Construction


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Blunting (2)

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cis-Repressed pQE Kan Vector Set  
QIAGEN

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cis-Repressed pQE Vector Set. For cis-repressed high-level expression of N-terminally 6xHis-tagged proteins. Kit contents: 25 ug each: pQE-80-L-Kan, pQE-81L-Kan, pQE-82L-Kan.
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C-Terminus pQE Vector Set  
QIAGEN

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C-Terminus pQE Vector Set. For high-level expression of C-terminally 6xHis-tagged proteins. Kit contents: 25 g each: pQE-16, pQE-60, pQE-70. Benefits: C-terminal 6xHis tag ensures that...
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Effectene Transfection Reagent (4 x 1 ml)  
QIAGEN

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Effectene Transfection Reagent. For DNA transfection of primary cells and sensitive cell lines. Kit contents: 4 x 1 ml Effectene Reagent, Enhancer, Buffer. for 160 transfections in 60 mm...
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EndoFree Plasmid Giga Kit (5)  
QIAGEN

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EndoFree Plasmid Giga Kit. For purification of up to 10 mg endotoxin-free advanced transfection-grade plasmid or cosmid DNA. Kit contents: 5 QIAGEN-tip 10000, Reagents, 5 QIAfilter...
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EndoFree Plasmid Mega Kit (5)  
QIAGEN

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EndoFree Plasmid Mega Kit. For purification of up to 2.5 mg endotoxin-free advanced transfection-grade plasmid or cosmid DNA. Kit contents: 5 QIAGEN-tip 2500, Reagents, 5 QIAfilter...
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HiPerFect HTS Reagent (2 x 1 ml)  
QIAGEN

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HiPerFect HTS Reagent. For high-throughput transfection of eukaryotic cells with siRNA or miRNA. Kit contents: Reagent for transfections in 80–200 x 96-well plates. Benefits: One-day,...
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His-Strep pQE-TriSystem Vector Set  
QIAGEN

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His-Strep pQE-TriSystem Vector Set . For parallel expression of His-Strep-tagged proteins in E. coli, insect, and mammalian cells. Kit contents: pQE-TriSystem His-Strep 1 and pQE-TriSystem...
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N-Terminus pQE Vector Set  
QIAGEN

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N-Terminus pQE Vector Set. For high-level expression of N-terminally His-tagged proteins. Kit contents: 25 g each: pQE-9, pQE-30, pQE-31, pQE-32, pQE-40. Benefits: pQE-30 vectors with MCS...
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PolyFect Transfection Reagent (1 ml)  
QIAGEN

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PolyFect Transfection Reagent. For fast and easy DNA transfection of standard cell lines. Kit contents: For 25–65 transfections in 60 mm dishes or 50–100 transfections in 6-well plates.. ...
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PolyFect Transfection Reagent (4 x 1 ml)  
QIAGEN

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PolyFect Transfection Reagent. For fast and easy DNA transfection of standard cell lines. Kit contents: For 100–260 transfections in 60 mm dishes or 200–400 transfections in 6-well plates..
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pQE-30 Xa Vector  
QIAGEN

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pQE-30 Xa Vector. For expression of His-tagged proteins containing a Factor Xa Protease recognition site. Kit contents: 25 μg pQE-30 Xa Vector DNA. Benefits: Easy cloning of a construct...
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pQE-TriSystem Vector  
QIAGEN

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pQE-TriSystem Vector. For parallel expression of His-tagged proteins in E. coli, mammalian cells, and baculovirus-infected insect cells using a single construct. Kit contents: 25 μg...
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QIAexpress Type IV Kit  
QIAGEN

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QIAexpress Type IV Kit. For high-level expression and one-step purification of N-terminally His-tagged proteins. Kit contents: 5 μg each: pQE-30, pQE-31, pQE-32 (N-terminal 6xHis). 10 ml...
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SuperFect Transfection Reagent (1.2 ml)  
QIAGEN

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SuperFect Transfection Reagent. For transfection of a broad range of eukaryotic cell lines with DNA. Kit contents: For 40 transfections in 60 mm dishes or 160 transfections in 12-well...
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TAGZyme DAPase Enzyme (2.5 U)  
QIAGEN

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TAGZyme DAPase Enzyme . For the removal of N-terminal His tags from proteins expressed using TAGZyme pQE vectors. Kit contents: For processing of approximately 50 mg tagged protein: 2.5...
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TAGZyme Kit  
QIAGEN

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TAGZyme Kit. For the removal of His tags from proteins with an intrinsic or an introduced DAPase stop point. Kit contents: For processing of approximately 10 mg tagged protein: 0.5 units...
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TAGZyme pQE Vector Set  
QIAGEN

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TAGZyme pQE Vector Set. For expression of proteins optimized for His-tag removal using TAGZyme enzymes. Kit contents: TAGZyme pQE-1 and pQE-2 Vector DNA, 25 g each. Benefits: Expressed...
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Exonuclease I  
Thermo Fisher Scientific

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Thermo Scientific Exonuclease I digests single-stranded DNA 3' to 5' (requires Mg2+ and a free 3'-OH). The nuclease is inactivated by heating at 80°C for 15 minutes.
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