Digital PCR, at its core, is simply a single molecule counting method that quantitatively measures absolute DNA and eliminates the need for standard curves. However, it has already shown potential to be a disruptive technology in many areas of diagnostics. The Second Annual Digital PCR event will bring together industry visionaries and early adopters to discuss digital PCR's capabilities, limitations, and future applications. Researchers will examine applications in cancer biomarker and rare mutation detection, non-invasive fetal DNA analysis, and infectious disease quantification, particularly in HIV. Novel digital PCR devices from startups and academic labs will be showcased. Other topics to be addressed include digital PCR integration with existing technologies, solutions for processing difficult samples as well as increasing sample throughput, and guidelines and best practices for digital detection.