An Improved SPE-LC-MS/MS Method for the Quantification of Bradykinin in Human Plasma Using the ionKey/MS System
27 August 2015
This study utilizes specifically designed blood collection techniques to inhibit bradykinin formation ex vivo, takes advantage of mixed mode solid-phase extraction (SPE) and use of the novel and highly efficient ionKey/MS System for selective, sensitive, and robust chromatographic separation, and quantification of the nonopeptide bradykinin. Accurate quantification of bradykinin in plasma is particularly challenging because it is present in low pg/mL levels, is rapidly metabolized, and is also artificially produced during blood sampling and sample preparation via proteolytic processes. Peptides in general are often difficult to analyze by LC-MS/MS, as mass spectrometer (MS) sensitivity is low due to the formation of multiple precursors and poor or overly extensive fragmentation.