Multiplexed Detection of Cytokine Cancer Biomarkers using Fluorescence RNA In Situ Hybridization and Cellular Imaging
31 May 2016
Download this method for profiling cytokine expression using fluorescence in situ hybridization (FISH). The described protocol offers a simplified and unique procedure, with RNA sensitivity that can be multiplexed to detect up to four target mRNAs simultaneously, in single cells. The method combines detection, imaging and analysis to provide a flexible and high-throughput procedure to detect important cancer-related cytokine biomarkers.