Determination of Carbohydrates in Urine by HPAE-PAD
2 Oct 2012
In this application note, two HPAE-PAD methods are compared for analysis of urine samples. The first method, using a Thermo Scientific Dionex CarboPac PA20 Analytical Column, minimizes eluent preparation with a Reagent-Free™ ion chromatography (RFIC™) eluent generator. The second method discussed takes advantage of the selectivity and high capacity of the Dionex CarboPac MA1 column. Use of this column allows mannitol to be strongly retained with minimal interferences. Both methods are shown to successfully determine carbohydrates in urine with good precision and minimal matrix effects from the samples. However, the first method saves time and reagent costs.