Application Note: Site-Specific Labeling of Antibodies for MicroScale Thermophoresis
27 January 2017
Site-specific conjugation of a dye to antibodies is useful for producing highly homogeneous material for use in downstream affinity experiments. This application note describes a labeling procedure that combines the selective reduction of antibody interchain disulfides and their labeling with maleimide-based chemistry. Different antibodies conjugated by this approach yield high-quality MST affinity data for interactions with both antigens as well as Fc receptors.