Application Note: Simultaneous Quantitation of Peptides and Phosphopeptides by capLC-ICP-MS using the Agilent 8800 Triple Quadrupole ICP-MS
18 Jan 2013
LC-MS/MS is used for the quantification of target proteins in pharma/biopharma and clinical research. This approach generally relies on the use of synthetic isotopically labeled proteins and peptides which are used as internal standards for the quantification of the corresponding target compound. This application note demonstrates the determination of proteins and peptides by the absolute quantitation of P and S heteroatoms using the Agilent 8800 Triple Quadrupole ICP-MS in MS/MS mass shift mode.