Application Note: Simultaneous Detection of GPCR Second Messengers in Living Cells
24 January 2017
Live cell assays allow monitoring of cellular responses, including functional information about the timing and location of events in the cell. This application note describes how second messengers relevant to the Gq signalling pathway can be detected in living cells using assays for DAG, PIP2 and Ca2+ by employing the CLARIOstar microplate reader. This article demonstrates how the CLARIOstar supplies high sensitivity detection of biosensors and can detect two distinct responses simultaneously.