Screening for Mitochondrial Biogenesis and Toxicity
1 February 2012

In this application note, adapted primary cultures of RPTC to exhibit in vivo levels of aerobic metabolism, to be non-glycolytic, and to retain high levels of differentiated functions were obtained. The XF96 Analyzer was used to measure mitochondrial respiration of the RPTC in real time. Changes in carbonylcyanide-4- (trifluoromethoxy) -phenylhydrazone (FCCP)-uncoupled respiration, a measure of electron transport chain (ETC) integrity and a sensitive measure of mitochondrial functional capacity, were used to quantify mitochondrial toxicity and biogenesis.

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