Application Note: Routine Determination of Glutathione and Glutathione Disulfide using a Novel Boron-Doped Diamond Electrode
15 July 2009

Although electrochemical detection is often used for the determination of tissue glutathione (GSH) and glutathione disulfide (GSSG) levels, this approach typically suffers from a number of issues related to chromatography, sample preparation and electrochemical detection. In this application note results indicate that the use of ESA's novel boron-doped diamond (BDD) thin-film electrode can prevent many electrochemical issues. The ability to operate at extreme potentials and resistance to contamination enables the electrochemical detection of thiols and disulfides to be considered routine.