Application Note: Quality Analysis of Virgin Olive Oils - Part 3
22 April 2013

This application note presents the analysis of chlorophyll degradation products pheophytin a, a’ and pyropheophytin a after sample preparation according to ISO 29841:2009(E). Pyropheophytin a was found in longer stored virgin olive oils as well as in partly refined olive oil. Fresh olive oil (about three months old) revealed only minor amounts of pyropheophytin a. Using the Agilent 1260 Infinity LC with fluorescence detection, virgin olive oil can be analyzed for proper storage conditions or potential refining processes.