Application Note: Processing Human Serum for Rapid and Reproducible N-Glycan Mass Profiling
28 May 2013
This application note describes a high throughput sample preparation procedure for global glycan mass
profiling. Microwave-assisted enzymatic N-glycan release followed by an automated solid phase extraction (SPE) purification procedure utilizing the Gilson GX-274 ASPEC™ System were employed to improve sample throughput and stabilize sample processing . A matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance (MALDI-FTICR) mass spectrometer was used to rapidly scan the glycan sample and capture high mass accuracy high resolution mass spectra.