Development and Validation of Automated Methods for Next-Generation Sequencing Library Preparation & Exome Capture
9 Aug 2013
Typical NGS workflows are very similar in they involve a number of manual liquid transfer steps and incubations. Although this is acceptable for small scale sequencing projects, the trend towards larger sequencing projects involving hundreds of samples presents a unique set of challenges with respect to throughput, cost and uniformity. These problems are compounded when involving associated processes such as exome capture or sample multiplexing. This scientific poster presents an automated protocol for preparing 1-24 Illumina libraries on a Janus 8-tip VariSpan workstation.