Application Note: Performing Oxygen Radical Absorbance Capacity Assays with Synergy™HT
9 January 2012

This application note presents a new way to measure total antioxidant capacity with the oxygen radical absorbance capacity (ORAC) assay. Using the BioTek Synergy HT Multi-Detection Microplate Reader the ORAC assay depends on free radical damage to a fluorescent probe, such as fluorescein. The degree of damage is indicative of the amount of radical damage.