Application Note: Odyssey Western Blot Blocker Optimization for Near-Infrared (NIR) Detection
14 July 2014
This document describes a method to determine optimal blocking conditions for NIR Western blot detection with the Odyssey family of Imagers. The speciﬁc lysate and antibodies used in your system will be evaluated in four diﬀerent blocking buﬀer systems. The buﬀering system will also be evaluated in this experiment.