Application Note: Obtaining Maximum Information with a Novel Workflow for in vivo Metabolite Detection and Identification using SYNAPT HDMS
6 March 2008

In vivo sample analysis for metabolite identification is extremely challenging due to the lack of radio labeled drugs in early discovery and the high level of endogenous biological non-drug-related interferences. As a result, there are no reference points for identifying xenobiotics a priori. Analysts rely heavily on personal experience and analytical strategies to detect and identify low-level metabolites. In principle, some of these problems may be reduced by utilizing an additional stage of separation. This separation stage is orthogonal to LC and mass spectrometric separations and occurs on an intermediate timescale between the two separations. The Waters® SYNAPT™ HDMS™ System provides this capability by combining high-efficiency ion mobility-based measurements and separations with tandem mass spectrometry. In this study, SYNAPT HDMS allowed the user to operate the system in either time-of-flight (TOF) mode or HDMS mode for maximum information.