Application Note: Screening for Use-Dependent Blockers of NaV1.5 Channels on the IonWorks Barracuda Automated Patch Clamp System
18 November 2013

This application note describes the design and validation of a cell based assay for the NaV1.5 channel, using the IonWorks Barracuda® Automated Patch Clamp System. A single concentration mock screen of known sodium channel blockers was set up to identify the location of the hits in a 384 compound plate. Follow-up experiments on the hits were then performed to identify use- and state-dependent blockade using a train of depolarizing pulses.

Download PDF >>PDF Icon

Read the full article...

  Become a member
  • Quick and complete access to all application articles

  • Download 10,000+
    applications and methods

  • Make informed buying decisions


Already a member?

Log in for full access to the article




Remember me