Application Note: Measuring of Macromolecular Interactions Using Composition‐Gradient Multi‐Angle Light Scattering (CG-MALS)
13 November 2016

In vivo, biomolecular therapeutics may participate in complex interactions with their protein or DNA targets that can reduce the efficacy of these products. This application note describes how composition gradient multi-angle light scattering (CG-MALS), automated by Calypso II hardware, can be used to quantify macromolecular associations between Cre recombinase and loxP DNA without requiring modifications that could interfere with the interaction. This is a simple, robust technique can be applied to a host of macromolecular interactions.