Application Note: Transcriptome Sequencing Using the Ion Proton™ System
19 Aug 2013
Understanding the structure, function, and organization of information within genomes is central to basic and translational research. With the advent of deep-sequencing technologies, it has become increasingly evident that transcription is an intricate and dynamic process involving a variety of RNA species, including short RNAs, polyadenylated RNA, and nonadenylated transcripts. Transcriptome sequencing is the most complete yet most cost-efficient method for identifying and quantifying RNA from multiple types of starting material. In contrast to microarray analyses that require a priori knowledge of transcripts in a cell for targets to be present on the microarray, transcriptome sequencing provides a hypothesisneutral approach to interrogating all the transcriptional content of a genome. This application note presents a method for transcriptome sequencing on the Ion Proton System from Life Technologies and compares the results to those obtained from microarrays and qPCR.