Application Note: Three Optimized Workflows for CpG Island Methylation Profiling
20 August 2013
Bisulfite DNA conversion is one of the most frequently used techniques for methylation studies because of its relative simplicity, whereas other methods are cumbersome and require significant optimization. The bisulfite conversion method allows precise analysis of methylation in a target region by converting all non-methylated cytosines into uracils, while methylated cytosines remain unchanged. This application note describes three workflows that provide an effective solution for methylation analysis with straightforward protocols.