Application Note: Highly Efficient and Robust Generation of Neural Stem Cells from Human Pluripotent Stem Cells Using a Serum-Free Neural Induction Medium
18 August 2013
Human pluripotent stem cells (hPSCs) including human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) are excellent sources for studies of cell fate specification, disease modeling and drug screening. In order to produce various neural cells from hPSCs, the induction to neural stem cells (NSCs) is the first important step. Conventional methods of NSC derivation from hPSCs typically involve in embryoid body (EB) formation or co-cultures with stromal cell lines, which have several disadvantages such as time-consuming and multi-procedures, low efficiency and variable quality of derived NSCs. This scientific poster demonstrates the use of a serum-free neural induction medium, which can convert hPSCs into NSCs in one week but without the time consuming, laborious processes of EB formation and mechanical NSC isolation.