Accurate and Sensitive Mutation Detection in Heterogeneous Cancer Research Specimens by castPCR Technology
23 May 2013
The discovery of pivotal genetic alterations and the understanding of their potential role in cancer is leading to remarkable successes in cancer research. Molecular methods such as DNA sequencing and conventional genotyping of tumor biopsies have advanced research in this field, but are limited in sensitivity due to stromal contamination and by genetic heterogeneity in cancer. This application note demonstrates how castPCR, a new rare mutation detection technology, has greater sensitivity, specificity and can thereby facilitate accurate mutation detection of heterogeneous cancer research specimens.