Determination of Amino acids by UHPLC with automated OPADerivatization by the Autosampler
19 Mar 2014
Amino acid analysis is a considerable application applied in research, clinical facilities and industrial processes. This application note descreibes a rapid and sensitive UHPLC method for the determination of amino acid concentrations and compositions. Using a KNAUER BlueShell core shell column and a high speed gradient method on the KNAUER PLATINblue UHPLC system, a complex mixture of 15 derivatized amino acids can be separated in less than 8 minutes.