Application Note: Kinetic Analysis of ß-Galactosidase Activity using the PowerWave™ HT and Gen5™ Data Analysis Software
26 March 2008

The determination of the enzyme kinetic parameters for newly discovered proteins is an important procedure in cellular and molecular biology. This application note describes the use of kinetic reading for the analysis of the bacterial enzyme, ß-galactosidase, using o-nitrophenol-ß-D-galactoside (ONPG) as the substrate.

Download PDF >>PDF Icon

Read the full article...

  Become a member
  • Quick and complete access to all application articles

  • Download 10,000+
    applications and methods

  • Make informed buying decisions

Already a member?

Log in for full access to the article

Remember me