Application Note: High-Throughput Analysis of Epigenetic Targets
23 December 2011

RapidFire/MS/MS was used to analyze SIRT1, SIRT2, and SIRT3 reactions at a sustained rate of approximately seven seconds per sample in this application note. The label-free method circumvents the need for non-native surrogate substrates and radioactive methodologies. RapidFire/MS/MS enabled direct detection of peptide reactants and facilitated the identification of kinetic parameters for accurate
IC50 determinations.