High Definition Data Directed Analysis: The Application of Quadrupole Ion Mobility Time-of-Flight Mass Spectrometry for Untargeted Proteomics Studies
23 Oct 2013

The increasing complexity of bottom-up proteomics challenges the capabilities of mass spectrometers to generate more and more detailed information. Instrument speed, sensitivity, and mass accuracy have all increased significantly over recent years, and novel LC/MS acquisition schemas and fragmentation mechanisms have been introduced. In this study, instrumental and application benefits are demonstrated for the identification of proteins and peptides in a new high definition data directed analysis (HD-DDA) mode.

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