Application Note: Gene Expression Proﬁling and Validation Using Agilent SurePrint G3 Gene Expression Arrays
22 June 2013
Gene expression analysis is currently carried out using three methods that provide complementary data: microarrays, RNA-Seq, and RT-qPCR. Microarrays provide comprehensive whole-genome coverage in single experiments, with rapid data acquisition and results at a low price per sample. RNA-Seq provides the most comprehensive data across all RNA transcripts present in a sample. However, RNA-Seq also requires the largest sample amounts, the most time, and the highest investment. RT-qPCR provides the fastest and the most cost effective solution for reliable expression analysis, but it is most suitable for experiments involving a small number of genes across a large number of samples. Download this application note to compare Agilent SurePrint G3 gene expression microarray data with the results achieved using RT-qPCR and RNA-Seq.