Defining Metabolic Phenotypes in Cancer Cells
3 May 2013
Malignant transformation of cells is often characterized by aerobic glycolysis, the conversion of glucose into lactate, despite the presence of oxygen. Cancer cell bioenergetic analyses have been limited to sequential, independent measurement of cellular respiration, glucose uptake, lactate production and mitochondrial function. While these assays measure parameters of bioenergetic function, they are time consuming, may require the use of radiolabeled reagents and often do not provide real-time data. This application note demonstrates how XF extracellular flux assays can measure both mitochondrial respiration (OXPHOS) and glycolysis simultaneously enabling a rapid and more comprehensive assessment of cellular bioenergetics.