Application Note: Multiplexed Antibody Characterization - Combinatorial Evaluation of Species Cross-Reactivity Using a High Capacity Flow (HCF) Approach
6 Feb 2013
The discovery and development of antibody therapeutics is a time-intensive and costly process that involves screening of large candidate libraries, functional characterization of lead molecules in vitro, and preclinical testing in animal models of efficacy and toxicity. In this application note it is shown that the multiplexing capabilities of the IntelliCyt HTFC® Screening System, which utilizes a flow cytometry detection modality, can be used to incorporate cross species reactivity of antibodies into the screening phase of the discovery process. This assay enables high content studies to be performed in a high throughput setting.