Rapid, Label-free Counting and Characterization of Live Embryoid Bodies
21 Jan 2013
Embryoid body (EB) formation is a common intermediate during the in vitro differentiation of embryonic stem cells and induced pluripotent stem cells into specialized cell types. The size distribution of EBs plays a significant role in the efficiency of differentiation and in production yields. In this application note it is shown that the Colony Counting: Embryoid Body Application on the Celigo cytometer from Brooks Life Science Systems provides an efficient, reproducible, automated method for assessing the number, size, and shape of live EBs within multi-well plates. In addition, the Celigo enables non-destructive monitoring of EB populations, thus they can be used following the analysis.