Application Note: Ruggedness Testing Including an Evaluation of Automation of a Cell- Based Bioluminescent TNFα Blocker Bioassay
30 January 2012

This poster describes a simple, homogeneous and robust bioluminescent TNFα blocker bioassay based on quantification of caspase 3 activity. The bioassay has good precision and accuracy and can be performed in one day. It uses U937 (human) cells which exhibit rapid response to TNFα. By developing and using U937 cells in single-use frozen, thaw-and-use format, scientists were able to remove variability arising from continuous cell culture.