Application Note: Live Cell Biosensor Assay Used To Interrogate GPCRs
21 November 2013
Functional, cell-based assays are essential to identify modulators of GPCRs, including those that signal via increases or decreases in cAMP. Several variants of the biosensor exist with different affinities for cAMP, some offering increased sensitivity and others offering increased dynamic range. In this application note the performance of the highest and lowest affinity variants are compared using automated methods and the highly-sensitive bottom reading capabilities of an HTS microplate reader. Sensitive detection of the 22F basal signal allows for use of this variant under a broad range of conditions, providing an assay with both superior dynamic range and sensitivity.