Application Note: Droplet Digital™ PCR: Ultra-Sensitive Rare Event Detection
6 November 2013

Droplet Digital PCR (ddPCR™) enables accurate, precise, and sensitive quantification of specific nucleic acid sequences. One of the advantages of ddPCR is that partitioning into droplets allows effective increase of rare target abundance within an individual compartment, and thus facilitates rare event detection (RED). In this application note it is demonstrated that the ddPCR system offers extra sensitivity for RED. As low as 0.0005% rare mutant and 0.00013% rare sequence were reliably detected.

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