Reducing PCR Reaction Times by Using Faster Ramp Rates, Modifying Programs and Changing the Reaction Mix
17 May 2010
In this application note the rapid ramp rate of the TC-PLUS was demonstrated. In the first experiment described above, there was little if any difference in yield or sensitivity between reactions run on the TC-PLUS and the slower TC-512. Modifications to the program and changing to a fast PCR mix can further shorten run times, although there may be some compromises in yield and sensitivity which would need to be taken into consideration if speed of the PCR is of primary importance over these factors.