Application Note: Simultaneous Analysis of GFP-Transgene Expression and Plant Cell Ploidy with the BD Accuri C6 Flow Cytometer
15 November 2012
The amount of DNA contained within a haploid nucleus of eukaryotic organisms is termed the C (constant) value. Monosomatic tissues containing mitotically active cells are characterized by cells with nuclear DNA contents ranging from 2C to 4C, depending on the position of the cells within the cell cycle. In polysomatic tissues, endoreduplication can occur, producing uninucleate cells having multiplicative DNA contents (2nC, where n = 1, 2 3…). In this application note the BD Accuri™ C6 flow cytometer was used to analyze nuclear C-values in combination with transgenic expression of a nuclear-targeted version of the GFP protein placed under the regulation of cell type specific promoters.