Application Note: Baculovirus Titration with the BD Accuri C6 Flow Cytometer
9 November 2012
The baculovirus expression vector system (BEVS) is increasingly popular among researchers as a tool for generating large amounts of recombinant protein in insect cells, which post-translationally process proteins in a manner similar to mammalian cells. Titration of the viral stock is an important step in optimizing recombinant protein expression. Recently, flow cytometry has emerged as a fast, accurate and efficient method to determine baculovirus titers. In this application note from BD Biosciences the BD Accuri™ C6 flow cytometer equipped with the BD CSampler™ Accessory is shown to be an ideal tool to assess the BEVS. The affordable BD Accuri C6 has a small footprint, and allows hands-free analysis of a 96-well plate.