Application Note: Detection of Intracellular Cytokines in T Lymphocytes using the BD FastImmune™ Assay on the BD FACSVerse™ System
16 October 2011

This application note describes a BD FastImmune CD4 3C assay for the detection of CD4+ lymphocytes producing IFN-γ upon antigenspecific stimulation with a cytomegalovius peptide pool (CMV pp65). Further, the BD-defined assay was customized to include three additional markers—CD3, CD8 and intracellular IL-2—to create a 6-color user-defined assay for characterization of polyfunctional CD4+ and CD8+ T lymphocytes.