Application Note: Automation of Multiplexed Cell-Based Assay to Measure Induction of Gene Expression and Activity of Cytochrome P450 Isoform 3A4
8 April 2010

This application note demonstrates the automation of a cell-based multiplexed assay to explore how a drug can induce gene expression and inhibit activity of the same isoform, creating a useful tool to measure the stimulatory and inhibitory effects of a drug. Activity and induction of gene expression of CYP3A4 were measured using a luminogenic, cell-membrane permeable 3A4-specific substrate and a luciferin based reporter gene assay.